The required volume can be reduced by thickening two or four sides of the bucket inside the walls. Factors such as sample characteristics, volume availability, concentration levels, and types of measurements to be taken should influence Cuvettes the decision when choosing the right bin for your applications. There are so many types of buckets available, even when the range is reduced to just those used for absorption measurements in the field of UV fish spectrophotometry.
Transmission extends from UV to mid-infrared light, ranging from 250 to 5,000 nm. Sapphire can withstand the extreme natural condition of some sample solutions and temperature fluctuations. As we all know, solutions with a higher concentration should be diluted or use a bucket with a shorter path length to simulate dilution. Known for the Beer-Lambert law, a light bucket range of 1 mm will allow the dsDNA concentration to be up to 1,000 μg/mL. In addition to the standard baking pad length of 10 mm, we also offer pad lengths ranging from 1 mm to 100 mm.
For measurement best practices, the bin should remain on the bin holder between measurements. If it is removed, it should be ensured that the bucket is always placed in the same direction on the bucket holder, i.e. with the label to the light source. This ensures that the optical effects are identical for both reference and sample measurements. Thanks to the excellent production tolerances of METTLER TOLEDO’s Excellence buckets, this preferred orientation is not necessary, as the absorption differences of 180 degrees are marginal. According to the Beer-Lambert law, the absorption is directly proportional to the length of the trajectory of the cuvettes and the concentration of the sample. Selecting an ideal path length (for example, from 1 mm to 5 cm) can eliminate the need for dilutions.
Table 1 gives an overview of the wavelengths used in their analysis and what they indicate. For reliability and best practices, UV-Vis spectroscopy experiments and measurements should be repeated. When repeating the examination of a sample, a minimum of three replication tests are generally common, but many more replicas are required in certain areas of work.
Regardless of the material selected, it should be noted that buckets, with the exception of clear plastic buckets, are fragile and must be handled carefully. A bucket is a small, transparent rectangular container used in spectrophotometric studies that comes in a variety of material options, quality levels, and dimensions. In the visible range of 320 to 2500 nm, glass buckets are cost-effective for measurements. Quartz material buckets are transparent across the UV spectrum and visible, from 190 to 2500 nm, making them an excellent choice for UV versus spectroscopic research. Smaller buckets can hold 70 microliters, while larger ones can hold 2.5 milliliters or more. The width determines the length of the path of light through the sample, which affects the calculation of the absorption value.
There are two, three, four or five polished bucket windows available. The most common type of bucket is square, with external dimensions of 12.5×12.5 mm. This size is suitable for sample volumes from the microlitre (sub-micro buckets) tosemi-micro to the milliliter range or even larger. The standard path length of a container is 10 mm and there are also buckets available that provide a shorter or wider light path. In UV-Vis spectroscopy, the wavelength corresponding to the maximum absorption of the target substance is chosen for analysis.
Because glass and quartz are reusable materials, they can be washed and maintained well, so that they can be reused for longer. Quartz has the ability to transmit more light and is more transparent. At the same time, glass and plastic buckets are not ideal for concentration and purity measurements.